Anonymous #1 7w

lol it’s weird cuz there’s a lot there that I think is so fire but it’s not exactly something anyone will read 😂 Writing a book for an audience of maybe 1

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Anonymous OP 7w

You wanna read 200 pages of statistics, computer science, and biology? Lmao there’s a really good bit on page 185 or so 😭

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Anonymous OP 7w

Oh wow, is your dissertation on Bioinformatics?

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Anonymous #5 7w

So basically, Petri dish experiments are generally qualitative ( mutant A grew, mutant B didn’t). I built software to measure colonies.. but also a whole pipeline to organize those measurements with the experiment context, and perform a lot of statistics

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Anonymous OP 7w

Chapter 1 introduces the software (QuantaColony). Chapter 2 is validation, I took images from existing papers that only made qualitative findings, I analyzed the photos , confirmed the findings with hard measures, then expanded on their analysis producing new results they had in those photos but didn’t report

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Anonymous OP 7w

So then, chapter 3 is using the software in my own experiments. I did the lab work and analyzed it, found some genetic interactions that I can l quantitatively measure And chapter 4 confirms that genetic interaction with molecular biology, in a lab. So that’s not the program, just more traditional lab stuff (yeast two hybrid)

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Anonymous #3 7w

Yeah, the degree is “Genetics, Genomics, and Bioinformatics”. I really did a lot of Bioinformatics, and a fair bit of genetics. No genomics really tbh

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Anonymous #4 7w

lol thanks! 200 more to go, I have a lot of data I still need to cover

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Anonymous OP 7w

I’m gonna glaze the fuck out of you rn and tel you that, despite me also being in grad school, I’m only able to grasp the very basics of what you just painstakingly explained. No pressure to expound unless you’re bored and want to discuss further

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Anonymous #5 7w

lol tldr this is the main thing. I built a tool to measure colonies. That creates lists of measurements. It’s also interactive. So an automatic algorithm detects stuff, but the user can tweak those results for improved accuracy

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Anonymous OP 7w

You might have 5 Petri dishes, each with different conditions that you want to test (for example, a temperature series) So you take photos of those experiments, use QuantaColony, and learn about the trends of colony sizes across those pictures

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Anonymous OP 7w

So a photo of a Petri dish might turn into this figure

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Anonymous OP 7w

Got a much better idea now, pretty damn cool.

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Anonymous OP 6w

That’s dope, best of luck!!! My dad, who is a biochemical engineer, did his on the oxidative damage of cancer on mammalian cells and I believe his was around 415 pages long. You got this :)

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